Protocols + Guides

Your protocols and step‑by‑step guides live here. Easy to navigate, and created to support you at every stage. Our goal is to keep things clear and easy, so you always feel supported in understanding your research peptide.

Preparing your peptides

Prep

Clean the work area. Flip the plastic caps from peptide vial and bacteriostatic water. Swab the vial stoppers with 70% isopropyl alcohol. 

Draw

Use a sterile syringe to draw the chosen amount of bacteriostatic water needed for reconstitution.

Inject

Slowly inject the bacteriostatic water into the peptide vial. Angle the stream down the inside of the glass. Gently swirl the peptide vial until dissolved (do not shake).  

Store 

Store peptides in the fridge around 36-46 degrees Fahrenheit. 

Injection Guidance

Injection Types: a quick overview

There are two common injection categories: subcutaneous (SQ) and intramuscular (IM). SQ injections go into the fatty layer just beneath the skin, while IM injections reach deeper into the muscle. SQ injections are generally gentler and often used for products that don’t require deep tissue absorption.

Most research peptides fall into the subcutaneous category, which is why you’ll see SQ referenced more often across our materials. 

Subcutaneous (SQ) Injection Guidance

1. Gather supplies. You will need alcohol prep pad, peptide vial, syringe, and sharps container for disposal.
2. Wash your hands thoroughly. Wipe the rubber stopper of the peptide vial with an alcohol swab and let it air dry.
3. Pull the plunger back to draw air into the syringe equal to your dose. Insert the needle into the vial, push the air in, then invert the vial. Slowly draw the peptide into the syringe. Tap the barrel to move air bubbles to the top and gently push them out.
4. Select a site with adequate fatty tissue and rotate sites for every injection to prevent scar tissue or lumps.
5. Abdomen: At least 2 inches away from the belly button.
6. Thighs: The front or outer portion of the thighs.
7. Use an alcohol swab to clean the skin in a circular motion and let it dry.
8. Use your non-dominant hand to gently pinch a 1-inch fold of skin and fatty tissue.
9. Hold the syringe like a pencil and insert the needle at a 90-degree angle (perpendicular to the skin). If you have very little body fat, use a 45-degree angle.
10. Push the plunger slowly and steadily until all peptide is delivered.
11. Pull the needle straight out. Do not rub the site; apply gentle pressure with a cotton ball or gauze if there is minor bleeding.
12. Immediately place the used needle and syringe into a puncture-resistant sharps container. 

Intramuscular (IM) Injection Guidance

1. Gather supplies. You will need alcohol prep pad, peptide vial, syringe, and sharps container for disposal.
2. Wash your hands thoroughly. Wipe the rubber stopper of the peptide vial with an alcohol swab and let it air dry.
3. Pull the plunger back to draw air into the syringe equal to your dose. Insert the needle into the vial, push the air in, then invert the vial. Slowly draw the peptide into the syringe. Tap the barrel to move air bubbles to the top and gently push them out.
4. Select your injection site.
5. Thigh is preferred for self-injection. Locate the middle third of the outer thigh, between the hip and the knee.
6. Hip is also a preferred site for adults. Place the heel of your hand on the hip bone, point your thumb toward the groin, and form a "V" with your index and middle fingers. Injection site will be in the center of the V.
7. Use an alcohol swab to clean the skin in a circular motion and let it dry.
8. Use your non-dominant hand to pull the skin and subcutaneous tissue roughly 1 inch to the side or downward.
9. Hold the syringe like a dart and insert it at a 90-degree angle with a quick, firm motion. Depress the plunger slowly and steadily (about 10 seconds per mL).
10. Pull the needle out at the same 90-degree angle and then release the skin. Do not rub the site; apply gentle pressure with a cotton ball or gauze if there is minor bleeding.
11. Immediately place the used needle and syringe into a puncture-resistant sharps container.
    

Research Peptide Protocols

ROA = Route of Administration, SQ = subcutaneous injection, IM= intramuscular injection

A quick note: information on this page is for research and educational purposes only. Always consult with a qualified healthcare professional before starting any new research protocol.